UseofSemiThinCryosectionsforLightMicroscopy.
Use of Semi-Thin Cryosections for Light Microscopy.Semi-thin sections can be obtained from frozen blocks of cryoprotected biological material by sectioning at -90°C. The advantages for using these sections for light microscopy are that the subcellullar morphology is preserved and the optical resolution is improved. Chemically fixed biological material is cryoprotected by immersion in 2.3 M sucrose and frozen onto spe......閱讀全文
Use－of－SemiThin－Cryosections－for－Light－Microscopy.
Use of Semi-Thin Cryosections for Light Microscopy.Semi-thin sections can be obtained from frozen blocks of cryoprotected biological material by secti
USE－OF－THE－LIGHT－MICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjus
Silver－Enhancement－...
實驗概要The method provides a silver enhancement protocol for immunoassay.主要試劑Prepare the following reagents fresh daily except for the citrate buffer.1.
Light－Microscopy
The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool
Measurement－with－the－Light－Microscope
Measurement with the Light MicroscopeYour microscope may be equipped with a scale (called a reticule) that is built into one eyepiece. The reticule ca
Use－of－a－Hemacytometer
A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the c
Use－of－Micropipets
A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identifie
胚胎和成年斑馬魚眼情的組織學準備
INTRODUCTIONThis protocol describes the histological preparation of embryonic and adult zebrafish eyes. The methods described here can be easily adapt
The－Effects－of－Ultraviolet－Light－on－the－Fertilization
Jill K. Flemming, Franklin & Marshall College, Class of 2001IntroductionThe objective of this project is to observe the effects of UV radiation on bot
USE－誘變實驗
? ? ? ? ? ? 實驗材料 帶 mutS 表型（例如 BMH71-18) 的轉化用大腸桿菌感受態 帶 mut+表型的轉化用大腸桿菌感受態 試劑、試劑盒
USE－誘變實驗
實驗材料 帶 mutS 表型（例如BMH71-18) 的轉化用大腸桿菌感受態帶 mut+表型的轉化用大腸桿菌感受態試劑、試劑盒 退火緩沖液貯存液合成緩沖液噬菌體 T4DNA 連接酶噬菌體 T4DNA 聚合酶或測序酶單一位點的限制性內切核酸酶瓊脂糖凝膠誘變引物選擇引物質粒 DNA儀器、耗材 70°C
USE－誘變實驗
本方法中，兩條寡核苷酸引物雜交到變性重組質粒 DNA 雙鏈的同一條鏈上。一條引物（誘變引物）攜帶一個擬引進靶 DNA 序列的突變，第二條引物攜帶一個能破壞質粒單一限制酶位點的突變。本實驗來源于分子克隆實驗指南（第三版）下冊，作者：〔美〕J. 薩姆布魯克 D.W. 拉塞爾。實驗材料帶 mutS 表型（
Zebrafish－whole－mou...
實驗概要Whole mount staining of Zebrafish embryos, now commonly used, requires extra steps to fix and permeabilize to ensure the egg membrane is perme
Use－of－Transmission－Electron－Microscopy
?Use of Transmission Electron MicroscopyOverviewA protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented.?MaterialZe
Use－of－the－GUS－Reporter－Gene
One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter act
CREATION－AND－USE－OF－YOUR－INFECTIOUS－VECTOR
實驗概要CREATION AND USE OF YOUR INFECTIOUS VECTOR實驗步驟Day 1? ? ? ? 1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scal
Use－an－IgG－antiIgM...
實驗概要This method involves coupling an IgG anti-IgM antibody to protein A or G beads (see method below). These beads can then be used in the normal
Creation－and－Use－of－Infectious－Virus－Vector
Creation and use of your infectious vector:Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired).The f
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia??Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are?analgesia?(pain relief),?amn
HOW－TO－USE－THE－COULTER－COUNTER－TO－COUNT－CELLS
1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum.Usually put 0.2 ml
Chick－or－Mouse－embr...
實驗概要The following procedure describes the procedure for whole mount staining of chick or mouse embryo’s. A similar procedure could be used for sta
Setup－and－use－of－a－twolaser－multiphoton－microscope
Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imagingDavid Entenberg,1 Jeffrey Wyckoff,1 Bojana Gligori
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo
Basic－Theory－and－Use－of－GCMS（一）
BASIC?THEORY?AND?USE?OF?GC-MSbyDr.?Eugenia?SobolevaContent1.??Introduction.2.??GC-MS?systems?and?components.3.??Vacuum?system3.1.??Rotary?pump3.2.??Di
Use－of－the－Bradford－Protein－Assay－in－a－Microtiter－Plate－Format
Introduction?The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change in
Basic－Theory－and－Use－of－GCMS（二）
For?ionisation?to?take?place?at?all,?chemical?reaction?between?the?sample?and?the?reagent?gas?must?be?exothermic.?The?grater?the?heat?of?the?reaction,
梅特勒托利多LabX－Light－滴定軟件
LabX Light 滴定軟件產品詳細說明 描述儀器控制功能：滴定儀控制在線曲線、測量值、一階導數和結果直接控制滴定儀的輔助功能：攪拌、饋液、樣品轉換器的轉動、電位測量每次分析后自動打印結果（用戶可自定義內容）自動將結果導出到本地或網絡硬盤（用戶可自定義內容） ? ??
雜散光(Stray－light－)對吸光度的影響
一、雜散光的重要性雜散光是紫外可見分光光度計非常重要的關鍵技術指標。它是紫外可見分光光度計分析誤差的主要來源, 它直接限制被分析測試樣品濃度的上限。當一臺紫外可見分光光度計的雜散光一定時, 被分析的試樣濃度越大, 其分析誤差就越大。ASTM 認為: “雜散光可能是光譜測量中主要誤差的來源。尤
可見光檢測器－visible－light－detector
可見光檢測器 visible light detector 又稱分光光度檢測器，是基于溶質分子吸收可見光的原理設計的檢測器。能夠直接采用可見光檢測的溶質不是很多，而且多數靈敏度也不高，但采用具有高摩爾吸光系數的有機試劑（配位體和螯合劑）作為衍生化試劑進行柱前或柱后衍生操作的衍生化光度檢測法是相當
Spectrophotometry
A spectrophotometer or colorimeter makes use of the transmission of light through a solution to determine the concentration of a solute within the sol