UseoftheBradfordProteinAssayinaMicrotiterPlateFormat
Introduction The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change in absorbance in Coomassie Blue G-250 upon binding of protein (Bradford, Anal. Biochem. 72: 248, 1976). Unlike many other assays, including the Lowry procedure, the Bradford assay is not susceptible to interference by a wide variety of chemicals present in sampl......閱讀全文
Use－of－the－Bradford－Protein－Assay－in－a－Microtiter－Plate－Format
Introduction?The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change in
Bradford－protein－assay
Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly
蛋白質定量
Quantitative Determination of Peptides by Sulfhydryl (-SH) Groups?New?(Contributed by David Van Horn, Dept. of Chemistry, UC Berkeley Greg Bulaj, Dept
Biorad－Protein－Assay:－Bradford
Biorad Protein Assay: BradfordStandards: 1 mg/ml BSA stock- dilute 1:10 to get 0.1 mg/ml BSAAdd To get H-2O20 μl 2 μg/ml 780 μl40 μl 4 μg/ml 760 μl60
Bradford－Protein－Concentration－Assay
Bradford Protein Concentration Assayversion 01/07/2001Abbreviations:mcg = microgramsmcL = microlitersBSA = bovine serum albuminO.D. = optical densityd
Bradford法蛋白定量（Bradford－Protein－Assay－）
Bradford Assay is a rapid and accurate method commonly used to determine the total protein concentration of a sample. The assay is based on the observ
ELISA－Protocol－(General－Guidelines)
實驗概要Sandwich ?enzyme-linked immunosorbent assays (ELISAs) involve attachment of a ?capture antibody to a solid phase support. Samples containing known
ELISA－Protocol－(General－Guidelines)
實驗概要Sandwich ?enzyme-linked immunosorbent assays (ELISAs) involve attachment of a ?capture antibody to a solid phase support. Samples containing known
Bradford－Assay
Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B
Bradford－Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
Bradford－–－Protein－Determination
Bradford – Protein DeterminationIntroductionA rapid and accurate method for the estimation of protein concentration. The technique is simpler, faster
夾心ELISA2
General?Protocol for the Sandwich ELISA method:Before the assay, both antibody preparations should be purified and one must be labeled.For most applic
Capture－ELISA
實驗概要Capture ELISA ?(also known as "sandwich" ELISA) is a sensitive assay to quantitate ?picogram to microgram quantities of substances (such as hormon
RAT/MOUSE－GROWTH－HO...
實驗概要This ?Rat/Mouse Growth Hormone ELISA kit is used for the non-radioactive ?quantification of Growth Hormone in rat or mouse serum, plasma, tissue ?
LOWRY－PROTEIN－ASSAY
The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al.,?J. Biol. Chem. 193: 265-
Protein－Assay－(Spectrophotometer)
Protein Assay (Spectrophotometer)Use BSA (bovine serum albumin) 1mg/ml stock solution (1ml Eppendorf tubes) for standard curve.Place 0, 2, 5, 10, 15,
BIURET－PROTEIN－ASSAY
BIURET PROTEIN ASSAYMATERIALSBiuret ReagentBovine serum albumin (BSA)Spectrophotometer and tubesPROCEDUREPrepare standard dilutions of BSA containing
Leaf－GUS－Assay
實驗概要a protocol for?Leaf GUS Assay?This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs there are
Dynamic－Flow－Assay－in－a－Parallel－Plate－Flow－Chamber
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
Measurement－of－Cell－Adhesion－Under－Static－Conditions
Many different molecules have been described to promote cell adhesion including several cell surface carbohydrate-binding proteins. Measuring cell adh
In－Vitro－Protein－Ubiquitination－Assay
Ubiquitination is one of the most important posttranslational modifications in all eukaryote organisms. Ubiquitin-activating enzyme (E1), ubiquiti
Angiotensin－Protein－Kinase－Assay
James Hardwick's angiotensin assay protocolThis specific procedure was developed to assay the activity of the Lck kinase expressed from a retrovir
Automated－Extraction－－Normalized－DNA－Buccal－Kit
實驗概要This section ?provides a general protocol for automated isolation of genomic DNA from ?human buccal cell swabs in a 96-well format using the Charg
新技術：InCell－Western－Assay2
III. Experimental ConsiderationsProper selection of microplates can significantly affect the results of your analysis, as each plate has its own chara
cAMP分析－cAMP－Assays
cAMP AssaysGouzel Karimova and Daniel LadantUnite Postulante de Biochimie des Interactions Macromoleculaires, Departement de Biologie Structurale et C
競爭性分析Epitope－Mapping實驗方法
ABSTRACTThe simplest way to determine whether two monoclonal antibodies bind to distinct sites on a protein antigen is to carry out a competition assa
ELISA
Gangliosides ELISA protocol?(Contributed by?pingsunjim)This protocol can be used for detection of gangliosides.Specific antibodies to gangliosides are
alamarBlue?－Cell－Viability－Assay－Protocol
實驗概要Cell health can be ?monitored by numerous methods. Plasma membrane integrity, DNA ?synthesis, DNA content, enzyme activity, presence of ATP, and c
細胞黏附和遷移
Cell Adherence Assay?(LTI)General and nice Protocol for cell adherence assay. Proteins are coated on microtiter plates and cells are added; after the
ELISA－Inhibition－Assay
ELISA Inhibition AssaySensitize a 96-well microtiter plate with purified antigen.Prepare a solution of the purified antigen of interest in phosphate b