HarvestinglymphocytesfromPeripheralBlood
Blood Harvest· Anesthetize mice with 50ml of Xylene-Ketamine(2:1) coctail· Pin animal to a board (use distal extremities) supine· Use 70% alcohol to moisten the abdomen and lower thorax· Make an midline incision using forceps and scissors from mid abdo......閱讀全文
Harvesting－lymphocytes－from－Peripheral－Blood
Blood Harvest·??????? Anesthetize mice with 50ml of Xylene-Ketamine(2:1) coctail·??????? Pin animal to a board (use distal extremities) supine·???????
Culture－of－Peripheral－Blood－Lymphocytes－for－Chromosome－Analysis
實驗概要Provide information about chromosomal abnormalities.實驗原理The ?blood cell karyotyping method was developed to provide information ?about chromosomal
Harvesting－Hematopoietic－Cells－from－Mice
Materials4 mice from each genotype4 Ly5 miceBuckets with wet ice 3xBucket with dry ice 1xDewar flask with liquid nitrogen100 mL beakers with 95% ethan
Peripheral－blood－“endothelial－progenitor－cells”
EPC Isolation and Characterization1.?EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf
Human－Peripheral－Blood－Mononuclear－Cell－Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained fro
FACS－Analysis－Using－Peripheral－Blood－Cells
FACS Analysis Using Peripheral Blood CellsCollect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) and mix
Isolation－of－peripheral－blood－mononuclear－cells－(PBMC)
1.?Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2.?PBMC were isolated by centrifugation over lymphocyte separation medium. 3
Preparation－Of－Peripheral－Blood－Cells－For－Chromosome－Analysis
實驗概要Lymphocytes ?are differentiated cells which normally do not undergo subsequent cell ?divisions. By culturing lymphocytes in the presence of a mito
DNA－Extraction－from－Blood
實驗概要The ChargeSwitch? ?gDNA Purification Kits allow rapid and efficient purification of ?genomic DNA from small volumes of human blood. After preparin
Separation－of－Platelets－from－Whole－Blood
PurposeThis protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow chamb
Serum－Separation－from－Whole－Blood
Serum Separation from Whole Blood1) Collect sample (preferably in glass tubes) and leave for 1 hour at 37°C to allow it to clot.2) Leave sample at 4°C
Protocol－for－Isolating－DNA－from－Blood－with－Nucleated－Red－blood－Cells
實驗概要DNA ?isolation from fish or avian blood sample can be difficult because it ?contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is ?de
DNA－Purification－from－Blood－or－Body－Fluids
實驗概要試劑盒，操作簡單，稍有點貴，還可以接受。實驗材料blood實驗步驟Protocol: DNA Purification from Blood or Body Fluids(Spin Protocol)This protocol is for purification of total (ge
Isolate－DNA－from－NRBC－Blood－from－Buccal－Swab－collected－with－filter－paper
實驗概要DNA ?isolation from fish or avian blood sample can be difficult because it ?contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is ?de
Isolation－of－liver－lymphocyte
Isolation of liver lymphocyte???Several lymphocyte subpopulations reside in the normal adult human liver. Liver lymphocytes mainly include a large n
Red－Blood－Cell－Lysis－Protocols
實驗概要BioLegend’s ?Red Blood Cell (RBC) Lysis Buffer (Cat. No. 420301) has been designed, ?formulated, and tested to ensure optimal lysis of RBCs in sin
細胞培養常規操作
常規操作(主要內容如下)·?????????Aseptic Technique·?????????Culture Vessels·?????????Cell Counting·?????????Primary Culture·?????????Maintenance of Cell Line?·??
紅細胞裂解的實驗方法步驟
IntroductionPrior to using lymphoid tissue cell suspensions for flow cytometric analysis and/or for?in vitro?functional assays, it is recommended to r
Method:－Lymphoblastoid－Cell－Lines－from－Frozen－Whole－Blood
Method: Lymphoblastoid Cell Lines from Frozen Whole BloodMay 31, 1990Rosalie VeilePurpose:Blood Samples can be stored frozen as a backup in case an LC
Isolation－of－PBMCs－from－patient－blood－and－buffy－coats1
1| Transfer heparinized venous blood of patient to plastic50-ml tubes and dilute with an equal volume of PH buffer.When sodium citrate has been used a
Vacuum/Spin－Protocol－for－Isolating－DNA－from－Blood－with－Nucleated－Red
實驗概要DNA ?isolation from fish or avian blood sample can be difficult because it ?contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is ?de
細胞遺傳學——染色體
Chromosome Staining and Banding Technique?(Primate Cytogenetics Network)Protocols for different staining method, each is in great detail.??Karyotype A
細胞組分和細胞器——染色體
Chromosomal DNA Prep : cultured cells/tissue samples?(Mike A Dyer)This protocol was developed for cultured cells but should be appropriate for dissoci
The－OP9DL1－System:－Generation－of－TLymphocytes－from－Embryonic2
Freezing ESCs?25. Passage the ESCs as described in Step 22. Resuspend the?cells in 3 mL of freezing medium for OP9 cells.?26. Aliquot?1 mL of cell sus
The－OP9DL1－System:－Generation－of－TLymphocytes－from－Embryonic3
Protocol 3: Fetal Liver-Derived HSC Differentiation on OP9-DL1 CellsDay 0: Initiation of Fetal Liver Co-culture52. Isolate liver tissue from eight to
The－OP9DL1－System:－Generation－of－TLymphocytes－from－Embryonic4
TROUBLESHOOTINGProblem:?The OP9 cells are more than 80%-90% confluent.Solution:?It is important when creating working stocks of OP9?cells for freezing
The－OP9DL1－System:－Generation－of－TLymphocytes－from－Embryonic1
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes?and?Juan Carlos Zú?iga-Pflücker1Sunn
巨噬細胞和單核白細胞
·?????????Lymphocyte Transformation?(Donis-Keller lab)Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from antico
Immunofluorescent－Staining－of－Mouse－and－Rat－Leukocytes
I. ProcedureHarvest cells from tissue, preparing a single cell suspension. Red blood cells may be removed by lysis or density gradient: Red blood cell
Blood－Smear:－Preparation－and－Staining
Blood Smear: Preparation and StainingReference:Davidson, I. and Henry J., Clinical Diagnosis by Laboratory Methods, I. Davidsohn and J. Henry, eds., W