BloodSmear:PreparationandStaining
Blood Smear: Preparation and StainingReference:Davidson, I. and Henry J., Clinical Diagnosis by Laboratory Methods, I. Davidsohn and J. Henry, eds., W.B. Saunders Co., Philadelphia 1974, pp. 135-137.Wintrobe, M.W., et al., Clinical Hematology, Lea & Febiger,Philadelphia, 1974, p. 26.Principle:A thin blood smear is stained with Wright-Giemsa stain to enable evaluation of white blood cell, red cell and platelet mor......閱讀全文
Blood－Smear:－Preparation－and－Staining
Blood Smear: Preparation and StainingReference:Davidson, I. and Henry J., Clinical Diagnosis by Laboratory Methods, I. Davidsohn and J. Henry, eds., W
SMEAR－PREPARATION
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria
Examination－of－a－Mammalian－Blood－Smear
Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the
Preparation－and－Staining－of－Paraffin－Sections
I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice animal by prescribed and ap
Preparation－and－Staining－of－Frozen－Tissue－Sections
I. Preparation of Frozen Sections for SectioningMaterials neeed:2-methylbutane (isopentane)Liquid NitrogenDry icePeel-Away?/sup> base moldsFrozen tiss
Preparation－of－fixed－embryos－for－immunocytochemistry－and－AP－staining
1. Transfer 50 ml of embryo cultures to centrifuge tubes. Spin at 1500 rpm for 5 minutes. Check that you can see a pellet of embryos at the bottom.Qui
Human－Peripheral－Blood－Mononuclear－Cell－Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained fro
Preparation－Of－Peripheral－Blood－Cells－For－Chromosome－Analysis
實驗概要Lymphocytes ?are differentiated cells which normally do not undergo subsequent cell ?divisions. By culturing lymphocytes in the presence of a mito
細菌檢測
Gram Staining (+\-)?(William H. Heidcamp)??Gram-Staining Procedure?(MEDIC, U of Texas)Very nice and detailed method description for Gram staining??Aci
細胞遺傳學——染色體
Chromosome Staining and Banding Technique?(Primate Cytogenetics Network)Protocols for different staining method, each is in great detail.??Karyotype A
RNA－analysis－on－nondenaturing－agarose－gel－electrophoresis
實驗概要RNA analysis on non-denaturing agarose gel electrophoresis實驗步驟1. The following gel electrophoresis conditions are recommended:- use 1X TAE buffer
RNA－analysis－on－nondenaturing－agarose－gel－electrophoresis
1. The following gel electrophoresis conditions are recommended:- use 1X TAE buffer instead of 1X TBE- use agarose gel in the concentration of 1.1%-1.
流式細胞儀技術專輯
Flow Cytometry Analysis?(Springer Lab, Harvard University)?Flow cytometry employs instrumentation that scans single cells flowing past excitation sour
巨噬細胞和單核白細胞
·?????????Lymphocyte Transformation?(Donis-Keller lab)Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from antico
流式細胞儀技術專輯
?最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯：?嗚咽分享到：??????Flow Cytometry Analysis?(Springer Lab, Harvard University)Flow cytometry employs instrumentation that scan
DNA電泳
DNA電泳（主要內容如下）??Preparation of Agarose Gel and Electrophoresis??Extraction of DNA From Agarose Gel??Extraction of DNA from Acrylamide Gels??DNA Marker?
Culture－of－Peripheral－Blood－Lymphocytes－for－Chromosome－Analysis
實驗概要Provide information about chromosomal abnormalities.實驗原理The ?blood cell karyotyping method was developed to provide information ?about chromosomal
Immunocytochemistry...
實驗概要The method provides a guideline procedure for staining of cell cultures using immunofluoresence.實驗步驟1. General procedure??? 1)?Coat coverslips wit
Double－immunofluore...
實驗概要We provide a protocol for immunofluoresent double staining incubating the antibodies together.In order to be able to examine the co-distributi
Application－Note:－Qdot?－Nanocrystal－Conjugates－in－Flow－Cytometry
實驗概要Researchers today ?are trying to maximize the information that they get out of flow ?cytometry experiments by looking at more parameters in a sing
BrdU－Labeling－Protocol
實驗概要The thymidine analog, 5-bromo-2-deoxyuridine (BrdU),is a common reagent used for cell proliferation assays and for the detection of apoptotic
Gram－Stain－(+\)
MaterialsColonies of bacteria from Exercise 12.2ToothpicksCrystal violetGram''s iodine95 ethanolSafraninMicroscopes with oil immersionProcedur
Gram－Stain－(+\)
實驗概要細菌的革蘭氏染色技術實驗材料Colonies of bacteriaToothpicksCrystal violetGram's iodine95% ethanolSafraninMicroscopes with oil immersion實驗步驟1. Before staini
Intracellular－Staining－Protocol
1.?Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min
Silver－Staining－Protocol
1x 40min - overnight?????50% MeOH, 12% Acetic Acid1x 30min??????????????????????????????????50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min?
Alkaline－phosphatase－staining
4.5.1.1 General informationEndothelial cells possess an endogenous alkaline phosphatase (AP) activity. The enzymatic activity of AP is not restricted
Fluorescent－Staining－of－Cells
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip.2. PB
Staining－Methods－for－cell
death Z. Xia 10/2/95The simplest way: trypan blue.?Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells;?P.I. (propidiu
Protein－Staining－Procedures
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Wholemount－staining－of－embryos
Fix embryos in formalin or MEMFA for one hour at room temperature with mixing. Rinse with TBS, replace with methanol, store at -20oC.Rehydrate by slow