LineageAnalysisofBlood
Materials:Capillary tubes1.5 mL Eppendorf microfuge tubes15 mL conical centrifuge tubes96-well V-bottom plates (Corning Costar 3894, from Fisher)Flow tubes (Falcon 352054)Reagents:0.5 M EDTA pH 8.0PBS + 0.5% heat inactivated FBS RBC Lysis Buffer 4.15 g NH4Cl 0.5 g NaHCO3 0.0186 g Disodium EDTA 200 mL H2OAntibodies: &n......閱讀全文
Lineage－Analysis－of－Blood
Materials:Capillary tubes1.5 mL Eppendorf microfuge tubes15 mL conical centrifuge tubes96-well V-bottom plates (Corning Costar 3894, from Fisher)Flow
FACS－Analysis－Using－Peripheral－Blood－Cells
FACS Analysis Using Peripheral Blood Cells Collect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) a
Preparation－Of－Peripheral－Blood－Cells－For－Chromosome－Analysis
實驗概要 Lymphocytes ?are differentiated cells which normally do not undergo subsequent cell ?divisions. By culturing lymphocytes in the presence of a m
Culture－of－Peripheral－Blood－Lymphocytes－for－Chromosome－Analysis
實驗概要Provide information about chromosomal abnormalities.實驗原理The ?blood cell karyotyping method was developed to provide information ?about chromosomal
Glycosphingolipid－analysis
1) Incubate cells with 1 μCi/ml of 3H-galactose for 72 hours.---> If treatment is for an extended period of time: treat in serum free media containing
Lipid－analysis
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer
Biosynthesis－and－Analysis－of－Bilins
The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first o
Analysis－of－Heme－and－Hemoproteins
Heme is perhaps the most ubiquitous cofactor found in nature and the most functionally diverse. Hemoproteins are involved in cell respiration (cy
Analysis－of－Oligosaccharide－Ligands
Analysis of Oligosaccharide Ligands?by High Performance Liquid Affinity Chromatography Analysis of Oligosaccharide Ligands by High Performance Liqui
Analysis－of－murine－BM
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis.? Techniques to do this are well established in hu
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
Flow－Cytometry－Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
Protocol－for－Isolating－DNA－from－Blood－with－Nucleated－Red－blood－Cells
實驗概要 DNA ?isolation from fish or avian blood sample can be difficult because it ?contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is
SQ－Blood－Mini－Protocol－for－1ml－Clotted－Blood
實驗概要 This protocol is designed for isolating genomic DNA from 1ml Clotted Blood. 主要試劑 Regents to be supplied by user 1. Proteinase K (20mg/ml) 2
SQ－Blood－Mini－Protocol－for－50ul－Clotted－Blood
實驗概要 This protocol is designed for isolating genomic DNA from 0.5-1 million cultured cells. For larger or smaller amounts of starting cell numbe
Genomic－Southern－Blot－Analysis
This chapter describes a detailed protocol for genomic Southern blot analysis which can be used to detect transgene or endogenous gene sequences
Analysis－and－Reconstitution－of－Phycobiliproteins:－Methods－for－the－...
Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment Reactions Phycobiliproteins are a homologous
Microtubule－Spindowns－for－Visual－Analysis
Microtubule spindowns for visual analysis can be performed on single microtubules or microtubules nucleated from axonemes/centrosomes. Although live
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis?I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA.?EmbeddingB.?CuttingC.?StainingII. Pr
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number
FACS－Analysis－of－ES－Cells
Isolate cells and dissociate to single cell suspension (can use Gibco Cell Dissociation Buffer, Accutase or Trypsin)Wash with 10% FBS/DMEM:F12For surf
Simultaneous－analysis－of－DNA－content
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques.??William Telford. Louis E. King and Pamela
PAM:－Prediction－Analysis－for－Microarrays
PAM: Prediction Analysis for MicroarraysClass Prediction and Survival Analysis for Genomic Expression Data Mining?Features:Performs sample classificat
SQ－Blood－DNA－Maxi－Protocol－for－410－ml－whole－blood
實驗概要 The E.Z.N.A.? ?SQ Blood DNA Kit is designed for isolating high molecular weight ?genomic DNA from fresh, frozen or anticoagulated whole blood.
Mouse－Compete－Blood－Counts
Materials:? 250 μL of fresh mouse blood in plastic tubes containing EDTA.? RBC lysis buffer?(388 mM NH4Cl, 29.7 mM NaHCO3, 25 μM Na2EDTA) 20.
DNA－Extraction－from－Blood
實驗概要The ChargeSwitch? ?gDNA Purification Kits allow rapid and efficient purification of ?genomic DNA from small volumes of human blood. After preparin
Rat－Blood－Collection－Protocols
實驗概要The procedure presented below describes a method for collecting rat blood.實驗步驟Rat should be fully anesthetized (e.g., unresponsive to toe pinch).1
Blood－Smear:－Preparation－and－Staining
Blood Smear: Preparation and StainingReference:Davidson, I. and Henry J., Clinical Diagnosis by Laboratory Methods, I. Davidsohn and J. Henry, eds., W
Examination－of－a－Mammalian－Blood－Smear
Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the
SQ－Blood－DNA－Midi－Protocol－for－500l3ml－whole－blood
實驗概要 The E.Z.N.A.? ?SQ Blood DNA Kit is designed for isolating high molecular weight ?genomic DNA from fresh, frozen or anticoagulated whole blood.