植物細胞培養（plantcellculture）技術概述
植物細胞培養技術就是為了某種目的而在細胞水平上對離體植物細胞或原生質體進行的一系列生物工藝學操作。它包括分離、培養、再生以及一系列相關的操作。就有用化合物的生產來說，它主要是指在無菌條件下通過懸浮培養植物細胞生產有用化合物的過程。理論與技術基礎：植物細胞全能性、微生物液體深層發酵系統、遺傳工程 意義： 1）不受地理、季節和氣候條件的限制； 2）節省土地，降低成本，生產周期短，可大大提高經濟效益； 3）可代替整體植株在工廠內連續生產所需產物； 4）可通過添加抑制劑等使生物合成按照人的意志進行； 5）可通過誘變篩選，獲得高產細胞株，并且可以進行特定的生物轉化獲得新的有用物質。 1. 發展與成果例證 1959年Tu1ecke和Nickell首次采用大體積〔20升〕的硫酸瓶進行了薔薇(Rosa sp．)莖段的細胞大量培養。 1967年Kaul和Staba......閱讀全文
植物細胞培養（plant－cell－culture）技術概述
植物細胞培養技術就是為了某種目的而在細胞水平上對離體植物細胞或原生質體進行的一系列生物工藝學操作。它包括分離、培養、再生以及一系列相關的操作。就有用化合物的生產來說，它主要是指在無菌條件下通過懸浮培養植物細胞生產有用化合物的過程。理論與技術基礎：植物細胞全能性、微生物液體深層發酵系統、遺傳工程
293細胞培養(cell－culture)技術
1、293細胞明顯適應酸性環境,pH值在6.9～7.1時,可順利貼壁生長, 換液時動作要輕。一般用高糖的DMEM培養基。2、傳代：倒去廢液，PBS洗一次（輕），用0.02%EDTA與0.25%Trypsin消化，生長良好細胞，培養瓶中輕搖,使之流遍所有細胞表面,即將其吸除或棄去消化30s，然后吸去，
MS－Plant－Tissue－Culture－Medium
Component mg/l in MS mg/l in stock Amount for
原代神經細胞培養方法－Neuron－Cell－Culture
1. Preparation of coverslips1.1- Mass cultureOur standard mass cultures are plated on astrocytes.??Those, in turn, are plated on glass coverslips pre-
Bacterial－cell－culture
MaterialsGlass culture tubes with metal caps and labelsGrowth medium, from media room or customizedGlass pipette tubesParafilmEquipmentVortexerFireboy
Maintenance－of－Cell－Culture
Maintenance of Cell CultureAuthor:?Nanci DonackiSource:?Contributed by Nanci DonackiDate Added:?Tue May 14 2002Date Modified:?Tue Apr 27 2004Abstract:
李家洋應邀在PLANT－CELL撰寫Reflections－on－Plant－Cell－Classics文章
The Plant Cell是植物領域的著名學術期刊，對植物學的發展起到了重要的引領作用。為慶祝創刊30周年，The Plant Cell雜志社邀請部分編委會成員及其他科學家對發表在該雜志的重要研究工作進行評述，以期增進對植物科學過去30年中重要研究進展的認識，激發對未知領域的探索。 李家洋研
Cell－Suspension－Culture－of－Arabidopsis
實驗概要Cell Suspension Culture of Arabidopsis?主要試劑10% (v/v) Household BleachCallus Induction Medium?? ? ?Gamborg's B5 Basal Medium?? ? ?0.5 g/liter M
stem－cell－culture－protocol
實驗概要stem cell culture protocol主要試劑cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
Cell－Culture－Media－and－Solutions
Antimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml fetal bovine serum, heat inactivated12.0 ml 200mM (100
Cell－Culture－Media－and－Solutions
Cell Culture Media and SolutionsDec. 18, 1990R. VeileAntimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml f
ES－Cell－Culture－and－Manipulation
MediaHigh glucose DMEM (-pyruvate, -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type, be sure!!)1X l-glutamine1X Penicillin/stre
Trabecular－cell－monolayer－culture
Originally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
中科院Plant－Cell揭示植物菌根共生能量來源
4月30日，國際學術期刊The Plant Cell在線發表了中國科學院上海生命科學研究院植物生理生態研究所王二濤研究組關于菌根共生的最新研究成果A H+-ATPase that Energizes Nutrient Uptake during Mycorrhizal Symbioses in
北京大學Plant－cell解析植物發育調控機理
近日來自北京大學、國家植物基因研究中心的研究人員在擬南芥中發現了一種新的轉錄遏制子TIE1，并證實TIE1通過將TCP轉錄因子與TOPLESS/TOPLESS-RELATED輔阻遏物連接到一起，調控了葉發育。相關論文發表在植物學權威期刊The Plant Cell雜志上。 領導這一研究
Human－B－cell－isolation－and－culture
實驗概要This protocol provides a general protocol for human B cell isolation and culture.實驗步驟B cell isolation1.?Donor blood was obtained with informed con
Isolation－and－culture－of－smooth－muscle－cell
1.Male Wistar rats of about 250g body wt were anesthetized with ether and were decapitated. ?2.Kidneys and thoracic aorta were removed, rinsed with
Primary－brain－cell－isolation－and－culture
1.?Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containing 20% (v/
Human－B－cell－isolation－and－culture
B cell isolation1.?Donor blood was obtained with informed consent under guidelines issued by the Medical Ethics Committee. 2.?Mononuclear cell fractio
Follicular－thyroid－cell－isolation－and－culture
Thyroid epithelial cells (also called follicular cells or principal cells) are cells of thyroid gland that are responsible for the production and
北京林大《Plant－Cell》解析植物細胞程序化死亡
2014年7月10日，國際植物學領域著名期刊《Plant Cell》（5年影響因子為10.125）在線發表了北京林業大學的一項最新研究成果“The Cysteine Protease CEP1, a Key Executor Involved in Tapetal Programmed Cell
上海交大張大兵《Plant－Cell》發表植物發育新文章
近日，知名期刊《Plant Cell》刊登了上海交通大學、諾丁漢大學等處的最新研究論文“ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis”。該研究表明，絨氈層特異性轉錄因子
The－Dos－and－Donts－of－Cell－Culture
Given below are a few of the essential "do’s and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipment (P
Aseptic－Technique－and－Good－Cell－Culture－Practice
AimTo ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross con
ES－Cell－Culture－and－Manipulation2
Picking ES cell clonesOne or two days before picking colonies prepare 24-well plates of feeders. You can also use alternate protocols that utilize 96-
ES－Cell－Culture－and－Manipulation3
Care and Handling of Feeder Layer CellsSTO/SNL cells are a derivative of the standard STO cell line. They are transformed with a LIF producing plasmid
PC12－Cell－Culture－and－Fusion
Cell CultureMaterials1. Falcon Primaria culture dishes.2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse se
A－primary－cell－culture－model－of－rabbit－uroepithelium
Isolation of Epithelial Cells from Rabbit Bladders?1.?Animal experiments were performed in accordance with the Animal Use and Care Committee.?2.?Urina
細胞培養（cell－culture）的一些常見問題與解答2
6、血清的有關問題：新生牛血清：新出生牛5天內取血制成小牛血清：小牛出生16周以內采血制成。胎牛血清：（進口血清）要求剖腹取胎制成。國內廠家往往不能做到，經常把出生2天以內采血稱為胎牛血清。根據血清的生產工藝及血紅蛋白、內毒素含量又分為：（1）特級胎牛血清：40納米過濾，內毒素含量≤10EU，血紅蛋
細胞培養（cell－culture）的一些常見問題與解答1
1、細胞傳代消化時所用胰酶濃度越高越好嗎？不見得！因為胰蛋白酶溶液的消化能力是和溶液的pH、溫度、胰酶濃度及溶液中是否含有Ca++， Mg++離子和血清等因素有關。通常情況下，pH 8.0， 溫度37 ℃其作用能力最強。另外，鈣、鎂離子及血清均能大大降低其消化能力。我們每次進行傳代消化前，一