ThermalInactivation
Thermal InactivationA simple, reversible way to a stop restriction reaction is by adding EDTA, which chelates Mg2+, thereby preventing catalysis. If further manipulations of the digested DNA are to be performed, the restriction endonuclease should be inactivated. Phenol/chloroform extraction and ethanol precipitation is an irreversible method for inactivation and removal of all restriction endonucleases; however, a m......閱讀全文
Thermal－Inactivation
Thermal InactivationA simple, reversible way to a stop restriction reaction is by adding EDTA, which chelates Mg2+, thereby preventing catalysis. If f
Serum－Thawing－－Heat－Inactivation
Serum Thawing & Heat Inactivation(Chris Cohick from JRH Biosciences catalogue)How to thaw serum:Serum that is stored at -10o C to -40o C is stable for
Serum－Thawing－－Heat－Inactivation
How to thaw serum:Serum that is stored at -10o C to -40o C is stable for extended periods of time. It is neither necessary or desirable to store serum
Map－Kinase－Inactivation－of－SMRT－Corepressor
Corepressors are coregulators that interact with transcriptional silencers in a variety of pathways such as cell proliferation, differentiation and ap
Thermal－Cycling－Profile－for－Standard－PCR
Initial denaturationIt is very important to denature the template DNA completely. Initial heating of the PCR mixture for 2 minutes at 94°–95°C is enou
TAILPCR（thermal－asymmetric－interlaced－PCR)簡介
在分子生物學研究中,基因克隆和分子雜交的探針制備等操作常需分離與已知DNA序列鄰近的未知序列，TAIL-PCR又叫熱不對稱交錯PCR，能夠較好地解決上述難題。該技術通過3個嵌套的特異性引物分別和簡并引物組合進行連續的PCR循環，利用不同的退火溫度選擇性地擴增目標片段，所獲得的片段可以直接用做探針標記
Basic－handson－course－in－thermal－analysis－software－(English)
METTLER TOLEDO is pleased to announce their series of one-day training courses, which are designed to familiarize users with thermal analysis theo
Gene－Inactivation－in－the－Cyanobacterium－Synechococcus－sp.－PCC－7002－and...
Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Const
熱導檢測器(thermal－conductivity－detector,TCD)結構
熱敏元件裝入檢測池池體中，制成熱導池，再將熱導池與電阻組成惠斯頓電橋。
熱導檢測器(thermal－conductivity－detector,TCD)應用
熱導檢測器是一種通用的非破壞性濃度型檢測器，理論上可應用于任何組分的檢測，但因其靈敏度較低，故一般用于常量分析。
熱導檢測器(thermal－conductivity－detector,TCD)原理
熱敏電阻消耗的電能所產生的熱與載氣熱傳導和強制對流等散失的熱達到熱動平衡，當載氣中有組分進入熱導池時由于組分的導熱系數與載氣不同，熱平衡被破壞，熱敏電阻溫度發生變化，其電阻值也隨之發生變化，惠斯頓電橋輸出電壓不平衡的信號，記錄該信號從而得到色譜峰。
Inactivation－of－Gsk3－by－AKT－causes－accumulation－of－bcatenin
Lipopolysaccharide (LPS) from XX bacteria induces a wide range of inflammatory responses, including the response of alveolar macrophages to bacteria i
DNA的酶學操作
DNA的酶學操作DNA Modifying Enzymes?(Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowledge
K通道的失活把控生物鐘于白天的興奮性
BK channel inactivation gates daytime excitability in the circadian clockJoshua P. Whitt, Jenna R. Montgomery& Andrea L. MeredithNature Communications
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（九）
The strongest and unique “acute” environment induced by cryo-thermal therapy stimulated the most effective anti-tumor immune response?As described
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（九）
3)?50 mm Disks.?Four 50 mm diameter, 2 mm thick disks were specially polished by Meller Optics to try to obtain strengthening disks. A previous po
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（一）
Interleukin-6 Induced Acute Phenotypic Microenvironment Promotes Th1 Anti-Tumor ImmunityTing Xue1, Ping Liu1, Yong Zhou2, Kun Liu1, Li Yang2, Robert L
膜片鉗常見問題解答（二）
1. input resistance是什么意思？如何測量？ 答：在電生理學中，“input resistance”指“輸入膜電阻（Rin）”。全細胞記錄時，給細胞膜施加一系列刺激方波（一般為超極化），在離子通道沒有開放的情況下測定跨膜電流，根據歐姆定律即可求出Rin。膜電阻（Rm）與膜輸入阻
TGFTIR－(TGAFTIR)熱重分析儀英文介紹
TG-FTIR (TGA-FTIR)Thermogravimetric analysis (TG) follows changes in mass of the sample as a function of temperature and/or time. TG gives charact
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（二）
Summary of ResultsThe important objective of Phase II research was achieved: to fabricate and test a prototype high power sapphire microwave windo
20點直播｜北京大學教授王瑋講述微系統熱管理
直播時間：2024年9月20日（周五）20:00-21:30直播平臺：科學網APPhttps://weibo.com/l/wblive/p/show/1022:2321325080680643297283（科學網微博直播間鏈接）科學網微博科學網視頻號北京時間2024年9月20日晚八點，iCANX T
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（十）
through a boundary conductance, h, on faces at z = +/- L.is controlled by the parameter hL/k, the Biot Number, where k is the thermal conductivity
利用Mastercycler－X50－PCR儀與epMotion系統實現高重復性的微...
利用Mastercycler X50 PCR儀與epMotion系統實現高重復性的微量體積PCR實驗Highly reproducible low Volume PCR with Mastercycler? X50 and epMotion? Arora Phang, Tim Schommartz,
BJUT－and－H.E.L.－Joint－LaboratoryCollaborative－Excellence－for－a－Bright－Future
By the end of 2023, the number of new energy vehicles in China reached 20.4 million； whether it's power batteries or new energy vehicles, China
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（十）
In this study, we found that “acute” response was strongly induced by cryo-thermal therapy with “acute” and high expression of a series of acute p
X失活的定義
中文名稱X失活英文名稱X inactivation定 義雌性成體細胞中兩條X染色體中的一條在遺傳性狀的表達上喪失功能的現象。應用學科細胞生物學（一級學科），細胞分化與發育（二級學科）
干式恒溫器的簡介
干式恒溫器取代了傳統的水浴加熱方式，新型的數控金屬加熱方式可廣泛應用于酶制劑反應，血清滅活試驗，Rh血樣研究，交叉配血，以及膽固醇的鑒定檢測等多個領域。Instead of tradional water heating method, these newly desinged ,digital
MN1基因突變與藥物因子介紹
腦膜瘤1（MN1）含有兩組CAG重復序列它被腦膜瘤中的平衡易位（4；22）破壞，其失活可能參與腦膜瘤32的發病機制[由RefSeq提供，2008年7月]Meningioma 1 (MN1) contains two sets of CAG repeats. It is disrupted by a
MN1基因編碼功能及結構描述
腦膜瘤1（MN1）含有兩組CAG重復序列它被腦膜瘤中的平衡易位（4；22）破壞，其失活可能參與腦膜瘤32的發病機制[由RefSeq提供，2008年7月]Meningioma 1 (MN1) contains two sets of CAG repeats. It is disrupted by a
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（四）
Flow cytometrySpleens were extracted after therapy and mechanically homogenized with gentle MACS Dissociator to obtain a single-cell splenocyte su