Decontaminationofcellsfromtheyeast
I Destroy yeast1. Aspirate medium and wash cell in PBS.2. Incubate cells at 37oC for 5 min in non-diluted antibiotic-antimycotic.3. Incubate cells at 37oC for 5 min in trypsin. Spin them down (100g, 5 min). DO NOT EXCEED 100g.II Secure cells from crosscontamination4. Slowly a......閱讀全文
Decontamination－of－cells－from－the－yeast
I???? Destroy yeast1.???? Aspirate medium and wash cell in PBS.2.???? Incubate cells at 37oC for 5 min in non-diluted antibiotic-antimycotic.3.???? In
Direct－PCR－from－Whole－Yeast－Cells:－Zymolyase－Method
Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pel
Plasmid－isolation－from－yeast
Pick colonies into 0.5ml of SD-Leu (or other appropriate SD medium)Vortex for 1minLeave to grow O/N for 18-24h at 30°C, 230-250rpm (best in 5ml bijou)
Method:－Removal－of－Yeast－Contamination－from－Lymphoblast－Cultures
Purpose:This method is advantageous for saving the occasional cultures that become contaminated. Yeast contaminated cultures will appear cloudy when s
Quick－and－Easy－Isolation－of－Genomic－DNA－from－Yeast
ProcedureTransfer 1.5 ml of liquid culture of yeast grown for 20 - 24 h at 30°C in YPD (1% yeast extract, 2% peptone, 2% dextrose) into a microcentrif
Dissociation－of－Cells－from－Primary－Tissue
實驗概要A ?common method to obtain single cell suspensions from primary tissue is ?enzymatic disaggregation. Expose the cells to enzymes for a minimal ?am
Harvesting－Hematopoietic－Cells－from－Mice
Materials4 mice from each genotype4 Ly5 miceBuckets with wet ice 3xBucket with dry ice 1xDewar flask with liquid nitrogen100 mL beakers with 95% ethan
Removing－cells－from－liquid－nitrogen
Put cryovial straight from storage and float in the 37篊 water bath- caution should be taken as on rare occasions vials can explode when heated up due
細胞培養常規操作
常規操作(主要內容如下)·?????????Aseptic Technique·?????????Culture Vessels·?????????Cell Counting·?????????Primary Culture·?????????Maintenance of Cell Line?·??
Eccles:Protein－Lysates－from－Cells－in－Culture
Cell Lysis Buffer5mL 0.1M Tris HCl pH 8 (10mM)0.44g NaCl (150mM)0.02g EDTA (1mM)0.5mL nonidet P40 (1% w/v)0.05g SDS (0.1% w/v)Make up to 50mL with MQH
Mesenchymal－progenitor－cells－derived－from－human－muscle
Harvesting traumatized muscle derived mesenchymal progenitor cells (MPCs)1.?The muscle tissue was dissected without contaminating by granulation, adip
Isolation－of－T4－cells－from－xenograft－tumors
1 Put mouse down?2 Wash skin by dipping mice into ETOH?3 Remove tumor and put into 5ml PBS containing Fungizone and Penstrep?4 Swirl around to rinse t
Isolation－of－human－multipotent－mesenchymal－stem－cells－from－second
Isolation of human multipotent mesenchymal stem cells from second‐trimester amniotic fluid?Culture of MSC from amniotic fluid1.?Twenty amniotic flui
Global－Expression－Profiling－of－RNA－from－Laser－Microdissected－Cells－at－...
Global expression profiling of RNA isolated from laser microdissected cells allows one to profile a specific set of cells allowing for enhanced se
Isolation－of－stromal－vascular－cells－from－human－adipose－tissue
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
Derivation－of－Dopaminergic－Neurons－(from－Human－Embryonic－Stem－Cells)
實驗概要Directed ?differentiation of specific lineages has been a focal point in the ?field of human embryonic stem cell (hESC) research. Cell replacement
Isolation－and－Culture－of－Multipotent－Stem－Cells－from－Human－Bone－Morrow
Bone marrow contains three types of stem cells:1.?Hematopoietic stem cells give rise to the three classes of blood cells that are found in the circu
Protocol－for－Isolating－DNA－from－Blood－with－Nucleated－Red－blood－Cells
實驗概要DNA ?isolation from fish or avian blood sample can be difficult because it ?contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is ?de
Isolation－of－Total－RNA－from－Animal－Cells－use－RNeasy－Mini－Kit
實驗概要Extract the total RNA from animal cells by using RNeasy Mini Kit (QIAGEN No.74104)?主要試劑SDS based extraction solution實驗步驟1. ?Harvest cells.1)?? Try
Ethidium－Bromide－Decontamination
N.B.:?Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide.Decont
酵母轉化
·?????????Yeast Transformation?(Gietz Lab)LiAc/SS-DNA/PEG Transformation·?????????Yeast Transformation?(Breeden Lab)LiAc method·?????????Large-Scale Y
酵母準備
Yeast DNA PreparationYeast Genomic Preparation? (Gottschling Lab)Rapid method for yeast genomic DNA isolation??Yeast DNA Preparation (rapid glass bead
Decontamination－of－Ethidium－Bromide－Solutions－and－Surfaces
WARNING: EtBr is toxic and mutagenic. Hypophosphorus and its solutions are\corrosive. Decontamination solution gives off a small amount of nitrogendio
線粒體熒光探針大全：TMRM，Mitotracker，JC1（4）
Nonyl Acridine OrangeNonyl acridine orange (A1372) is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a useful probe
酵母轉化的幾種方法
Modified Yeast Transformation?Inoculate cells from an overnight culture into 50 ml YEPD and incubate at 30°C with shaking. Typically, add 0.1 to 0.2 m
酵母雙雜交系統
·?????????Yeast Two-Hybrid System?(Finley Lab)This is one of the most comprehensive and detailed guide to yeast two-hybrid system technique with intro
Yeast－Nuclei－Isolation
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
蛋白質相互作用
Interaction Trap/Trap Two-Hybrid System·?????????Yeast Two-Hybrid System?(Finley Lab)This is one of the most comprehensive and detailed guide to yeast
Performing－a－hunt－by－interaction－mating
AbstractWhen more than one bait will be used to screen a single library, significant time and resources can be saved by performing the interactor hunt
Yeast－Media
YEPD (non-selection)-1% yeast extract-2% peptone-1.5% agar (if needed for plates)After autoclaving add glucose to 2% by adding 100 ml of 20% solution