SilverAcetateAutometallography(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for light microscopical and ultrastructural autometallographic (AMG) studies. Specific methods were developed to demonstrate endogenous zinc pools in synaptic and secretory vesicles; exogeneous mercury, silver bound as sulfide or selenide crystals, in lysosomes; and tissue-b......閱讀全文
Silver－Acetate－Autometallography－(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for l
Silver－Enhancement－...
實驗概要The method provides a silver enhancement protocol for immunoassay.主要試劑Prepare the following reagents fresh daily except for the citrate buffer.1.
Silver:－Lysate－for－Western
Grow cellsHarvestSpin downWash with PBSSpin down enough cells to collect a 50-100 μL cell pellet in a FastPrep tubePrepare lysis buffer (beforehand) a
Silver－Staining－Protocol
1x 40min - overnight?????50% MeOH, 12% Acetic Acid1x 30min??????????????????????????????????50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min?
Silver:－Colony－PCR
Zymolyase Solution:Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store aliquots
Silver:－TimeLapse－Microscopy
Pad Preparation1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages of a
SSR－GEL－and－Silver－Staining－Protocol
I.?EQUIPMENT:DNA sequencing unit (35 x 45 cm) & 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking platformsH
Protein－Staining－Procedures
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
SDI檢測儀XFsilver
SDI檢測儀/污染指數儀/污染指數測定儀 型號：XF-silver 指數（SDI）值，也稱之為FI（Fouling Index）值，是水質指標的重要參數之一。它代表了水中顆粒、膠體和其他能阻塞各種水凈化設備的物體含量。通過測定SDI值，可以選定相應的水凈化技術或設備。 在反滲透水處
銀染(silver－staining)操作規程
實驗原理：在堿性條件下，用甲醛將蛋白帶上的硝酸銀（銀離子）還原成金屬銀，以使銀顆粒沉積在蛋白帶上。染色的程度與蛋白中的一些特殊的基團有關，不含或者很少含半胱氨酸殘基的蛋白質有時候呈負染。銀染的詳細機制還不是非常清楚。 試劑：乙醇、冰醋酸、乙酸鈉、硫代硫酸鈉、硝酸銀、碳酸鈉、甘氨酸或EDTA.Na
SDI檢測儀XFsilver
SDI檢測儀/污染指數儀/污染指數測定儀 型號：XF-silver 指數（SDI）值，也稱之為FI（Fouling Index）值，是水質指標的重要參數之一。它代表了水中顆粒、膠體和其他能阻塞各種水凈化設備的物體含量。通過測定SDI值，可以選定相應的水凈化技術或設備。 在反滲透水處理
10mol/L乙酸胺（ammonium－acetate）配制方法
將77.1g乙酸胺溶解于水中，加水定容至1L后，用0.22um孔徑的濾膜過濾除菌。
8mol/L乙酸鉀（potassium－acetate）配制方法
8mol/L乙酸鉀（potassium acetate）：溶解78.5g乙酸鉀于足量的水中，加水定容到100ml。
3mol/L乙酸鈉（sodium－acetate）配制方法
溶解40.8g的三水乙酸鈉于約90ml水中，用冰乙酸調溶液的pH至5.2，再加水定容到100ml。
醋酸纖維薄膜電泳（cellulose－acetate－membrane－electrophoresis...
實驗原理醋酸纖維薄膜電泳是用醋酸纖維薄膜作為支持物的電泳方法。它具有簡便、快速、樣品用量少，應用范圍廣，分離清晰，沒有吸附現象等優點。目前已廣泛用于血清蛋白、脂蛋白、血紅蛋白、糖蛋白、多肽、核酸、同工酶及其他生物大分子的分析檢測，是醫學和臨床檢驗的常規技術。本實驗以醋酸纖維素為電泳支持物，分離各種血
常用試劑配制－4
Phytohemaglutinin (PHA)Available as kidney bean lectin. It is typically used as a stock solution of 10-20 g/ml in balanced salt solution. For tissue c
低背景的蛋白質銀染(silver－staining)方法
我們做蛋白質電泳的人都知道，銀染色很靈敏，有很強的說服力，但通常膠背景較深，不易掃描。在這里介紹一下低背景的蛋白質銀染方法。Step Reagent Time/minFix 50%EtOH, 12%HAC, 0.1%HCHO, 38%H2O 60Rinse 50% EtOH 5 min, 3 tim
PCR－clean－up－using－3M－sodium－acetate－and－chilled－absolute－ethanol
For each 20 ul of PCR product, prepare the following mixture in 1.5 ml Tube.2 ul of 3M sodium acetate (NaOAc) pH 4.540 ul chilled absolute ethanol.Tra
醋酸纖維素薄膜(cellulose－acetate－film)分離血清蛋白
原理 采用醋酸纖維素薄膜為支持物的電泳方法，叫做醋酸纖維素薄膜電泳。醋酸纖維素，是纖維素的羥基乙酰化所形成的纖維素醋酸酯。將它溶于有機溶劑（如：丙酮、氯仿、氯乙烯、乙酸乙酯等）后，涂抹成均勻的薄膜則成為醋酸纖維素薄膜。該膜具有均一的泡沫狀的結構，有強滲透性，厚度約為120微米。 醋酸纖
Two－Dimensional－Gel－Electrophoretic－Analysis－for－the－Human－Plasma－Proteome
OverviewThis protocol is a detail description of the laboratory procedure in performing 2D gel electrophoresis for illustrating the protein profile of
醋酸氟孕酮（Flurogestone－acetate）ELISA檢測試劑盒使用說明
試劑盒只能用于科學研究，不得用于醫學診斷醋酸氟孕酮（Flurogestone acetate）ELISA檢測試劑盒使用說明書檢測原理試劑盒采用雙抗體一步夾心法酶聯免疫吸附試驗（ELISA）。往預先包被醋酸氟孕酮（Flurogestone acetate）抗體的包被微孔中，依次加入標本、標準品、H
2－Dimensional－Gel－Electrophoretic－Analysis－for－Chicken－Egg
Overview?? ? This protocol is a detail description of the procedure in performing 2D gel electrophoresis for illustrating the protein profile of the w
2007年度ScientificComputing雜志“讀者選擇獎”獲獎名單揭曉
“讀者選擇獎”是由Scientific Computing雜志主辦，每年評選一次。讀者從軟件產品的質量、可靠性、易操作性、技術支持和價格等幾方面考慮，從候選名單中選出他們中意的軟件。獎項涉及以下幾個領域的軟件：生物信息學、化學、色譜學、數據采集、數據管理、工程學、制圖、成像、數據接口、光譜學、數學、
蛋白質檢測
·?????????Protein detection?(Aberdeen's Lab)The method used to locate the proteins following 2D-PAGE depends on the nature of the original sample.
加州大學鐘聲團隊：用“一滴血”檢測胞外RNA液體活檢方法
細胞外RNA（exRNA）作為一種新型液體活檢標志物，因其廣譜性及多樣性近些年引起了科學家的廣泛關注。此前研究發現，包括miRNA在內的小exRNA與某些疾病的臨床治療反應相關，但關于其他類型exRNAs的存在情況及其臨床相關性研究仍十分有限。此外，血清中的exRNA經過提取步驟后，含量往往非常
MetalEnhanced－Immu...－（一）
實驗概要The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monola
Autoradiography
MaterialsH-Thymidine, specific activity of 2.0curie/millimoleOnion sets, jars and toothpicksAlcohol-acetic acid fixativeMaterials for feulgen reaction
蛋白質電泳
蛋白質電泳（主要內容如下）One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel?Gel StainingRecipesOne-Dimensional SDS-PAGE·??????
組織學——染色
Hematoxylin and Eosin Staining of Tissue for LCM?(Arcturus)???Immunohistochemical Staining (IHC)?(Arcturus)For optimal LCM from IHC samples, it is nec
常用試劑配制－5
Sodium citrate (MW 294.10)0.09 MDissolve 2.65 grams of sodium citrate to a final concentration of 100 ml with water.Sodium citrate/formaldehyde (for s